The 60-hour crunch: realities and possibilities of working with X-ray free electron lasers

Written by: Eilidh McClain

Edited by: Olivia Pifer Alge, Mena Davidson, Kristen Loesel, and Jennifer Baker 

Illustrated by: Jacquelyn Roberts

Start of experiment. Shift one. 3 hours in.

Cuckoo! “Scan finished.”

We hear the announcement that a scan is finished, sitting in a control room at Hamburg’s European X-ray Free Electron Laser. I wasn’t expecting it the first time; it’s such a silly little sound to use in a serious scientific lab. I began to associate it with success in the experiment we were conducting—another successful data collection scan under our belts. The sound of people typing diligently on computers and the gentle hum of discussion between the roughly ten scientists in the small room provides a rather calming backdrop to the experience. With each experiment, I am amazed at all that goes into setting up measurement scans. The control room computer screen is littered with endless computer screens, each monitoring important parameters of the experimental setup in the other room. The decisions are made by the visiting scientists, coming from multiple labs all over the world, but the equipment is monitored and handled by the three to four resident scientists at the facility.

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Steve Henikoff: Genome-Wide Mapping of Protein-DNA Interaction Dynamics

Live blogger: Eilidh McClain

Editors: Paul Dylag and Jennifer Baker

This piece was written live during the 7th annual RNA Symposium: From Molecules to Medicines, hosted by the University of Michigan’s Center for RNA Biomedicine. Follow MiSciWriters’ coverage of this event on Twitter with the hashtag #umichrna.

In response to multiple external factors, chromatin in chromosomes is able to dynamically shift in order to facilitate gene regulation. Gene expression is altered in part by the use of RNA-protein interactions within the chromatin. However, study of these interactions features many experimental requirements that are not optimized for studying chromatin dynamics as a whole and its role in gene regulation. Dr. Steve Henikoff and coworkers at the Basic Sciences Division of the Fred Hutchinson Cancer Center have tackled this RNA-protein interaction problem by developing new and powerful tools for studying those interactions. Now that these tools have been developed, they can provide interesting insights to the role of chromatin dynamics in regulation of gene expression and silencing with relative ease compared with previous methodology.

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